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. 2013 Oct 20;1(5):e00105. doi: 10.1002/phy2.105

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© 2013 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society

Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.

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(A) Reduced immunostaining expression of eNOS in pulmonary arteries from hypoxemic COPD patients. Images on top: eNOS immunostaining of proximal arterial rings with magnification ×5. *, control (n = 7) versus nonhypoxemic COPD (n = 7); Φ, control versus hypoxemic COPD (n = 6); ¥, nonhypoxemic COPD versus hypoxemic COPD; NS, no significant difference. (B) Reduced gene expression of eNOS measured by RT-PCR in pulmonary arteries from hypoxemic COPD patients. *, control (n = 7) versus nonhypoxemic COPD (n = 7); Φ, control versus hypoxemic COPD (n = 6); ¥, nonhypoxemic COPD versus hypoxemic COPD; NS, no significant difference. (C) Protein expression of eNOS, total RhoA, and GTP-RhoA in pulmonary arteries measured by Western blot and pull-down assay. *, control (n = 7) versus nonhypoxemic COPD (n = 7); Φ, control versus hypoxemic COPD (n = 6); ¥, nonhypoxemic COPD versus hypoxemic COPD; NS, no significant difference.