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. 2013 Oct 20;1(5):e00109. doi: 10.1002/phy2.109

Figure 5.

Figure 5

MMP-9 activity in plasma and lung. (A) MMP-9 pro-form and activity levels in plasma measured by gelatin gel zymography increased after hemorrhagic shock (HS) regardless of whether the intestine was flushed or nonflushed. *P < 0.005 by paired t-test between pre- and postsamples. ‡P < 0.05 by t-test between post-HS-F and post-HS-NF samples. (B) MMP-9 band corresponding to the sum of the MMP-9 dimer, pro- and active forms of MMP-9 after shock measured by immunoblot. §P < 0.05 by Mann–Whitney test. (C) Protein levels of MMP-9 in the lung also increase after HS regardless of intestinal flush. (D) The activity bands corresponding to MMP in the lung measured by gelatin gel zymography. Bar graphs show mean ± SD. N = 6 rats/group. §P < 0.025 by Mann–Whitney test. **P < 0.025 by Mann–Whitney test. (E) Corresponding activity levels detected by immunoblot showing the 125 kDa band corresponding to MMP-9 (samples were not reduced). Gelatinase activity by zymography of isolated neutrophils depicts three distinct bands for MMP-9, pro-MMP-9, and MMP-9 dimer at different dilutions (left).