Figure 3.

Quantitative real-time PCR confirmation of five genes identified by RNA-Seq as differentially expressed in muscle of gravid fish versus sterile fish. Differential expressions of four genes related to lipid metabolism (el, ehhadh, acca2, and hadhb) and an isoform of the gapdh gene were confirmed by qPCR. All genes showed statistically significant changes that are consistent with the RNA-Seq results (P < 0.05). * and ** indicate the P values <0.05 and <0.01, respectively, (LSMeans + SEM, N = 10). 2N and 3N indicate diploid and triploid fish, respectively.