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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Endocr Relat Cancer. 2013 Oct 24;20(6):833–848. doi: 10.1530/ERC-13-0164

Figure 5. The effect of overexpression of either WT MENIN or MEN1 disease-related variants on MAFA protein expression.

Figure 5

Following overnight glucose starvation (hour 0), INS-rβ cells overexpressing WT or variant MENIN after a doxycyclin treatment (dox −/+) were incubated in 11 mM glucose for 1 to 4 h as described in Fig. 2B. Under the Ctrl conditions, the cells were maintained in 5 mM glucose medium for the entire experiment. (A) IB of MAFA in INS_WT/B7 cells, a representative cell clone overexpressing WT MENIN. (B) IB of MAFA in INS_A160T/E4 and INS_A541T/I3 cells, representative cell clones overexpressing variant MENIN. The image is representative of at least four experiments on two independent cell clones for WT and variant MENIN proteins.