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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Endocr Relat Cancer. 2013 Oct 24;20(6):833–848. doi: 10.1530/ERC-13-0164

Figure 6. The effect of overexpression of either WT MENIN or disease-related variants on target genes mRNA levels.

Figure 6

Total RNA was extracted in parallel with the experiments shown in Figure 5. The expression levels of MafA, Ins2 and Slc2A mRNA were measured by real-time RT-qPCR as described above in INS_WT/B7 cells (A), INS_A160T/E4 (B) and INS_A541T/I3 (C) cells. The experiments were repeated four times. The relative quantity of each mRNA is indicated as a percentage of the respective control value fixed to 100% (i.e mRNA value for cells without overexpression of MENIN (no doxycyclin treatment) maintained in 0.1 mM glucose). *Statistical significance was defined as p<0.1. **Statistical significance was defined as p<0.05.