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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Methods. 2013 Aug 22;64(2):10.1016/j.ymeth.2013.08.008. doi: 10.1016/j.ymeth.2013.08.008

Figure 2.

Figure 2

Preparation and characterization of MBs. (A) Maleimide-MBs are coated with anti-Fcspecific antibody and anti-EpCAM antibody in a two-step process. (B) Resulting MBs have a broad size distribution, as measured with Countess™ cell counter software. (C) MBs float soon after redispersion. (D) Microscope images of the resulting MBs after staining of anti-EpCAM IgG with Alexa 488-labeled secondary antibody. Inset shows cropped image of a MB with characteristic “dentate” fluorescence. Size bar 20 μm.