hCNS-SCns transplantation at 9 or 60 DPI does not contribute to development of allodynia or hyperalgesia. Analyses were conducted by repeated-measures two-way analysis of variance (ANOVA). von Frey allodynia testing (A) revealed that no significant two-way interaction effects were found between the number of forepaw and hindpaw withdrawals in the 60 DPI hCNS-SCns cohort over time (black brackets), and no significant differences were observed between the vehicle and the transplant group at any time point of analysis (preinjury or 2, 7, 11, or 14 weeks post-transplantation [WPT]; Bonferroni post hoc test, p > .05). No significant two-way interaction effects were found in Hargreaves hyperalgesia testing (B) between forepaw and hindpaw withdrawal time in the 60 DPI hCNS-SCns cohort over time (black brackets), and no significant differences were observed between the vehicle and the transplant group at any time point of analysis (preinjury or 2, 7, 11, or 14 WPT; Bonferroni post hoc test, p > .05). At 14 WPT, CatWalk gait analysis revealed no significant differences in hindpaw maximum intensity (one-way ANOVA; p > .05) or in the duty factor (one-way ANOVA; p > .05) between the vehicle and the hCNS-SCns cohorts transplanted at 9 DPI (C) or 60 DPI (D). Error bars indicate SEM. Abbreviations: DPI, days postinjury; hCNS-SCns, human central nervous system-derived neural stem cell; NS, not significant; w, weeks.