Figure 6.

Correlations between von Frey and Hargreaves sensory behavior testing and the total proportion of human SC123⁺ astrocytes in the 9 DPI and 60 DPI transplant cohorts at 14 weeks post-transplantation (WPT). No relationship was found between the proportion of human SC123⁺ astrocytes in the 9 DPI human central nervous system-derived neural stem cell (hCNS-SCns) cohort and either the number of forepaw (A) and hindpaw (B) withdrawals in von Frey testing, or the time until forepaw (C) and hindpaw (D) withdrawal in Hargreaves testing at 14 WPT (Pearson r = 0.2, two-tailed t test; p > .6; r = 0.1, p > .8; r = 0.2, p > .6; and r = −0.4, p > .4, respectively). Although no histological or sensory behavioral evidence of development of neurological pain syndromes was found in any of the hCNS-SCns transplant cohorts, a positive correlation was found between the number of forepaw withdrawals in von Frey testing and the proportion of human SC123⁺ astrocytes (E) in the 60 DPI hCNS-SCns cohort at 14 WPT (Pearson r = 0.8, p < .02). No relationship was found between the proportion of human SC123⁺ astrocytes in the 60 DPI hCNS-SCns cohort and either the number of hindpaw withdrawals (F) in von Frey, or the time until forepaw (G) or hindpaw (H) withdrawal in Hargreaves at 14 WPT (Pearson r = −0.3, p > .6; r = 0.003, p > .99; and r = −0.004, p > .99, respectively). xy scatter plots show individual data points (dots) and curve fit with ±SEM (dashed lines). If all animals in a cohort exhibited a sensory behavioral value of 0 (gray line), for statistical analysis the values of two randomly selected animals were labeled as 0.001. Abbreviations: DPI, days postinjury; NS, not significant.