Figure 3. Wdpcp is required for cilia recruitment of Sept2, Mks1, and Nphp1.

(A–D) Ift88 is localized correctly to the basal body and ciliary tip in the rare cilium formed in Wdpcp^Cys40 mutant MEFs. (E–H) Nphp1 is normally found at the transition zone (E, and arrowhead in F), but in Wdpcp^Cys40 mutant MEFs, Nphp1 is mislocalized in the basal body (G, H). (I–L) Sept2 is normally found in the transition zone and sometimes in the ciliary axoneme in control MEFs (I, and arrowhead in J), but is absent from the cilium of Wdpcp^Cys40 mutant MEF (K, L). (M–P) Mks1 is normally found in the transition zone of the cilium (M, N), but it is absent from the cilium of Wdpcp^Cys40 mutant MEF (O, P). (Q–T) Wdpcp is localized to the transition zone in both control (Q, R) and Mks1 mutant MEFs (S, T). All panels are at the same scales, and the scale bar in (A) is 2 µm.