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. 2013 Oct 26;5(6):404–415. doi: 10.1093/jmcb/mjt039

Figure 4.

Figure 4

Growth suppression by ACAT2 inhibition in Huh7 xenograft tumors. (A–D) Effects of the ACAT2-specific inhibitor on Huh7 xenograft tumor growth and intracellular sterols. Huh7 xenograft tumors were established in nude mice and treatments with specific inhibitors (T2i or T1i) were performed as in A. Tumor volumes were assessed and calculated on the indicated days (B) and, at the end of the experiment, tumors were dissected, weighed (C) and determined for oxysterol contents, expressed as fold changes to the total oxysterol content from tumors in control mice receiving vehicle (D). (E and F) Effects of ACAT2 knockdown on Huh7 xenograft tumor growth. Different RNAi Huh7 stable cell lines were used to establish xenograft tumors in nude mice. Tumor volumes were assessed on the indicated days (E) and tumors were dissected for size and weight (F). The average tumor volume was expressed as fold change to that on Day 0 (B) or that of control RNAi Huh7 group on Day 13 (E). Statistical analysis was performed with repeated-measures two-way ANOVA (Bonferroni post hoc test; B and E, mean ± SEM, n = 11) and one-way ANOVA (Bonferroni post hoc test; C and F, mean ± SD, n = 11; D, mean ± SD, n = 8). *P < 0.05, **P < 0.01, ***P < 0.001.