Table 2. Chromosomal damage observed in E and NE individuals using uniform staining in cultures with aphidicolin vs standard culture.
| Culture with aphidicolin |
|
Standard culture |
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Exposed |
Non-Exposed |
p-value |
|
Exposed | Non-Exposed |
p-value | |||||||
| Total individuals | 14 | 14 | 14 | 14 | |||||||||
| Total metaphases analyzed | 1441 | 1410 | 1500 | 1463 | |||||||||
| Total metaphases with lesions (%) | 947/1441 (65.7) | 699/1410 (49.6) | 0.0141 | 12/1500 (0.8) | 8/1463 (0.5) | 0.4775 | |||||||
| Total metaphase with structural alterations No./total (%) | 46/1441 (3.2) | 16/1410 (1.1) | 0.0376 | 15/1500 (0.01) | 1/1463 (0.07) | 0.0594 | |||||||
| Chromosomallesions (% in 100 cells) | 1864/1441 (129.3) | 1216/1410 (86.2) | 0.0231 | 13/1500 (0.9) | 10/1463 (0.7) | 0.6455 | |||||||
| Gaps | 1007 | 623 | 6 | 6 | |||||||||
| Chromatid gap | 496 | 346 | 5 | 6 | |||||||||
| Chromosome gap | 511 | 277 | 1 | 0 | |||||||||
| Breaks | 857 | 593 | 7 | 4 | |||||||||
| Chromatid break | 239 | 107 | 5 | 2 | |||||||||
| Chromosome break | 618 | 487 | 2 | 2 | |||||||||
| Apparent structural chromosomal alterations (%) | 63/1441 (4.4) | 19/1410 (1.4) | 0.0239 | 24/1500 (1.6) | 1/1463 (0.07) | 0.0791 | |||||||
| Balanced | |||||||||||||
| Translocations | 7 | 2 | 0 | 0 | |||||||||
| Imbalanced | |||||||||||||
| Deletions | 12 | 0 | 0 | 0 | |||||||||
| Acentric fragments | 11 | 3 | 16 | 1 | |||||||||
| Imbalanced translocations | 9 | 0 | 0 | 0 | |||||||||
| Dicentric translocations | 15 | 12 | 0 | 0 | |||||||||
| Rings | 7 | 1 | 2 | 0 | |||||||||
| Markers | 1 | 1 | 6 | 0 | |||||||||
| Duplications | 1 | 0 | 0 | 0 | |||||||||
| Total chromosomal damage (%) | 1927/1441 (1.33) | 1235/1410 (0.87) | 0.0161 | 37/1500 (0.025) | 11/1500 (0.07) | 0.1080 | |||||||