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. 2013 Nov 26;8(11):e82478. doi: 10.1371/journal.pone.0082478

Figure 4. Knockdown of galectin-3 changes the mRNA expression patterns in Caco-2 cells after epirubicin treatment.

Figure 4

(A) The effect of treatment with CTR, scrambled shRNA, shGal-3, transfection with a galectin-3 overexpression vector (overGal-3), and/or Epi for 48 h on the mRNA expression level of galectin-3, GSK-3β, and β-catenin as determined by real-time PCR. *, P < 0.05 compared with CTR; +, P < 0.05 compared with shGal-3; &, P < 0.05 compared with Epi; #, P<0.05 compared with overGal-3. (B) The effect of treatment with CTR, scrambled shRNA, shGal-3, overGal-3, and/or Epi for 48 h on the mRNA expression level of cyclin D1 and c-myc as determined by real-time PCR. *, P < 0.05 compared with CTR; +, P < 0.05 compared with shGal-3; &, P < 0.05 compared with Epi; and #, P<0.05 compared with overGal-3. (C) The effect of treatment with CTR, scrambled shRNA, shGal-3, overGal-3, and/or Epi for 48 h on the mRNA expression levels of the MDR pump-related genes encoding MDR1, MRP1, and MRP2 as determined by real-time PCR. *, P < 0.05 compared with CTR; +, P < 0.05 compared with shGal-3; &, P < 0.05 compared with Epi; #, P<0.05 compared with overGal-3.