Figure 4. Protocol for voltage-dependent modification of hNav1.4.
The experiment involved ten oscillations between two voltages: 900 ms at the depolarizing test voltage (voltage sensors outward, fast-inactivated state) and 1100 ms at the hyperpolarizing holding potential (HP) (voltage sensors inward, recovered from inactivation). During these oscillations the channel was perfused with toxin/MTS solution. The sampling of the currents took place during perfusion with control solution after ten of the previously described oscillations (every 20 s) during a 40 ms depolarization to –20 mV allowing the channel to open and inactivate.