Figure 5. Kinetics of R3C modification with externally applied MTS in absence and presence of toxin.

The solid lines are calculated with the time constants (◆) LqhαIT absent, τ_MTS = 1.5 min; (▲) LqhαIT present (50 nM), τ_MTS = 17 min and F_S = 1-exp(-t/τ_MTS). Holding potential = –140 mV, test potential –90 mV. The protocol is described in the text (Figure 4). [MTSET] = 20 μM.