Figure 1. Substantial levels of APLP2 are present in Ewing sarcoma cell lines. Lysates of the Ewing sarcoma cell lines A4573, RD-ES, and TC-71 were immunoblotted with a polyclonal antiserum specific for APLP2 or with an antiserum for actin (as a loading control). MDA-MB435s (melanoma cell line) and 721.221 (B lymphoblastoid cell line) were included as controls expressing known high or low (respectively) levels of APLP2. The white line indicates the position of an intervening lane that was removed from the figure. The data shown are representative of results from 2 experiments that included these 3 Ewing sarcoma cell lines analyzed together and are representative of over 10 experiments that included various other groups of Ewing sarcoma cell lines.