Table 1.

Values of the spatial frequency, expressed as a fraction of Nyquist frequency, at which Lorentzian functions – fitted to the Fourier amplitude spectra of “empty” images – fall to 0.5, depending upon the type of detector and the electron energy. The sum of a single Lorentzian function plus a constant y-axis offset was fitted to the Fourier amplitude spectra (see Figure 1A for an example). Please refer to Figure S1 for fitted amplitude spectra for all other examples listed in this table. Values given in parentheses are the reciprocal of the respective values of the spatial frequency, i.e. the distance in number of pixels at which the nearly exponential linespread function falls to e⁻¹. Recall that Nyquist frequency is 1/(2 pixel).

	TVIPS F416 4kx4k 15.6 μm pixel	Gatan US4000 4kx4k 15.0 μm pixel	FEI Eagle 2kx2k 30 μm pixel	Gatan K2 4kx4k 5 μm pixel
80 keV	-	-	0.43 (4.7 pixels)	-
120 keV	0.44 Nyquist (4.5 pixels)	0.32 (6.3 pixels)	-	-
200 keV	-	0.27 Nyquist (7.4 pixels)	-	-
300 keV	-	-	0.35 (5.7 pixels)	0.43 (4.6 pixels)