Figure 8. Proliferative response after light lesions.

Proliferating cells are labelled by BrdU (red). A, G: Untreated control shows weak autofluorescence in photoreceptors. B–D: Clusters of BrdU+ cells are indicated by white arrowheads. E–F: Overview images showing proliferating cells at 5 dpl in the characteristic lesion areas (between arrows). G–J: Time course after a diffuse light lesion. K, L: Quantification of BrdU+ cells in the whole retina per cell layer normalized to 1 mm retinal length. Most proliferating cells are found in the INL and ONL at 3 dpl. BrdU+ cells found in the ganglion cell layer and the ciliary marginal zones are indicated as ‘Other’. Error bars indicate SEM. Scale bar represents 20 µm (A–D, G–J) or 500 µm (E, F).