Figure 3. Nuclear reprogramming induced remodeling of the bioenergetic infrastructure. iPS cell-specific proteome is revealed in representative silver-stained, broad pH range (3–10) large format two-dimensional gels (A). Two-dimensional gel electrophoresis consistently resolved on the order of 550 protein species in fibroblast and iPS cell extracts, with densitometric quantification identifying a subset of 97 significantly altered protein spots (21% of the total resolved) following nuclear reprogramming, with significance determined by Student t test (P < 0.05) (B).