Figure 2. miR-10b locus is involved in breast tumorigenesis. (A) In metastatic tumors, miR-10b is overexpressed by Twist transcription. This feature is closely related to the activation of invasive program through downregulation of HOXD10 and consequently overexpression of several cell migration repressor such as RhoC, uPAR, and MMP14.¹⁹ (B) In primary breast tumors miR-10b* is downregulated by CpG island hypermethylation. This feature leads to upregulation of its target genes, such as BUB1, PLK1, and CCNA2 and, in turn, to tumor proliferation.²⁷