Figure 5. Wound-healing defects in adult L1 Tg animals and migratory deficiency in L1 keratinocytes (A) Representative ear images of a WT mouse and a L1 Tg mouse (both ~6-mo-old). (B) Wound-healing defects in 3-mo-old L1 Tg. Shown on top are representative H and E images (×40) depicting that the WT epidermis completely re-epithelialized and closed the wound 1 wk after injury, whereas the L1 Tg epidermis showed disorganized and incompletely healed wound. Shown at the bottom are gross images of wounds in WT and Tg animals 12 d post epidermal abrasion. Note incomplete wound closure in the L1 Tg epidermis. The H and E and gross images shown are representative of a total 11 (for WT) and 9 (for L1 Tg) animals analyzed. (C) IHC staining for Ki-67 and K6 as short-term markers of proliferation and differentiation, respectively. Shown are images 48 h after wounding. Original magnifications, ×100. (D) Graph depicting the number of cells that migrated into scrape wound area 12 h post-scrap (left) and microscopic images of newborn keratinocyte cultures at 0 and 12 h post-scrape (right).