Figure 6. Stem cell defects in L1 Tg keratinocytes. (A) Schematic showing mouse IFE, hair follicle, and the resident stem cell populations. (B and C) Reduced numbers of CD34+α6+ bulge stem cells in the L1 Tg hair follicles. Hair follicles were prepared from 8-wk-old mouse dorsal skin and CD34+α6+ bulge stem cells analyzed by flow cytometry. (B) Bar graph presentation of CD34+α6+ cells. (C) Representative flow cytometry plots of CD34 and a6 staining. (D and E) Reduced numbers of Lrig1+ stem cells in the L1 Tg hair follicles. Hair follicles were prepared from 6–8-wk-old mouse dorsal skin and Lrig1+α6+ bulge stem cells analyzed by flow cytometry. (D) Bar graph presentation of Lrig1+ stem cells. (D) Representative flow cytometry plots of Lrig1 and α6 staining. (F) qPCR analysis of 16 genes in keratinocytes prepared from 6–8 wk WT or L1 Tg animals.