Figure 5. The FACT complex contains SSRP1 and SPT16 mRNAs. HeLa cell lysates were used for immunoprecipitation (IP) with anti-SSRP1 or IgG (non-specific isotype-matched control) antibodies. RNA isolated from the immunoprecipitated complexes was used as the template for RT-PCR with primer sets specific for SSRP1, SPT16, or IL-8 mRNAs or 18s rRNA.(A)Total cell extracts were used for immunoprecipitation.(B)Nuclear cell extracts were used for immunoprecipitation. In (B), ‘no-template’ controls (first lane in each set of three) and two different sets of SPT16 primers (SPT16-1and SPT16-2) were used. *Indicates the position of the primers on the gel. Note that an18S product was detected with both anti-SSRP1- and IgG-immunoprecipitated complexes, suggesting non-specific binding.