Figure 1. Acute treatment with rapamycin alters polysome profile in mouse liver tissue. (A) Example liver polysome profile. Polysome gradients contain peaks representing an insoluble fraction, free ribosome subunits (40s and 60S), and active ribosomes (R) separated by the amount of ribosomes tethered to mRNA. (B) Acute rapamycin treatment involved a single injection of vehicle or rapamycin (8 mg/kg). Tissue was harvested 1 h after injection. (C and D) representative liver polysome profiles from vehicle (C) and rapamycin (D) treated mice. Arrows indicate 40 and 60S peaks. (E) Quantification of polysome peaks from vehicle and rapamycin-treated liver tissue. *P < 0.05; 2-way ANOVA, Bonferroni post hoc test. For both groups, n = 8.