Fig 3.

PhageφC31ointegrase mediated stable integration of the NF-κB-luc reporter. A total of 10 μg of pattB-NF-κB-Fluc was delivered to mice with and without 20 μg of PphiC31oby hydrodynamic injection. On day 11 and 30, the luciferase activity was monitored after LPS challenge. Imaging on day 20 was performed without LPS challenge and served as a baseline control for the day 30 imaging data. (A). Luciferase activities after LPS challenge of the transfected mice (n=4) on day 11, 30 (B) and day 80 and 300 (C, D) were quantified and shown. The results are representative of 2 experiments. Nested PCR was used to detect the junction between the attB site of pattB-NF-κB-Fluc and mpsL1 at 30 days after the DNA injection (E).