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. 2013 May 10;99(3):514–524. doi: 10.1093/cvr/cvt113

Figure 3.

Figure 3

KLF2 knockdown impaired ECFC tube formation and migration. siRNA knockdown of KLF2 in ECFCs. (A and B) Q-PCR and western blot analysis of mRNA and protein levels of KLF2 and ECFC differentiation marker TM, eNOS, ICAM-1 CD34, and c-kit. β-actin was an internal control. (C) FACS analysis of ECFCs stained with antibodies against FITC-conjugated anti-human c-kit, FITC-conjugated anti-human CD34, FITC-conjugated anti-human CD31, PE-conjugated anti-human VE-cadherin. Data are representative of three separate experiments. (D–F) Control and knocked down cells were then cultured with or without VEGF (25 ng/mL) for 24 h. Tube-formation (D) and cell migration (E and F) assays were performed as described in Figure 1. Data are mean ± SD from three independent experiments, each performed in triplicate (*P < 0.05; **P < 0.01).