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. 2013 May 10;99(3):514–524. doi: 10.1093/cvr/cvt113

Figure 4.

Figure 4

Overexpression of KLF2 drove ECFCs towards differentiation into ECs. ECFCs were infected with adenovirus overexpressing KLF2 (Ad-KLF2) or control vector (Ad-null) for 24 h. (A) Q-PCR analysis of mRNA levels of KLF2 and EC markers. (B) Western blot analysis of protein levels of KLF2, eNOS, and ICAM-1. (C) FACS analysis of ECFCs stained with antibodies against FITC-conjugated anti-human c-kit, FITC-conjugated anti-human CD34, FITC-conjugated anti-human CD31, PE-conjugated anti-human VE-cadherin. Data are representative of three separate experiments. (D) Tube-formation assay was performed. Data are mean ± SD from three independent experiments, each performed in triplicate (*P < 0.05; **P < 0.01).