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. 2013 May 10;99(3):514–524. doi: 10.1093/cvr/cvt113

Figure 6.

Figure 6

Neovascularization by BMPC in mice was enhanced by KLF2 overexpression. BMPC from C57BL/6 mice were infected with adenovirus encoding KLF2 (Ad-KLF2, 50 multiplicity of infection) or with siRNA knockdown of KLF2 and mixed with Matrigel. Cells were subcutaneously injected into C57BL/6 mice, which were maintained for 7 days. Implants were sectioned and stained with haematoxylin and eosin (H&E) (A), anti-VE-cadherin (VE-Cad) (B), and anti-FLAG (Flag) (C). Arrows indicate vessel-like structures. Images are representative of implants from six different animals. Microvessel density in Matrigel implants was quantified by counting luminal structures containing erythrocytes. Data are mean ± SD from three independent experiments, each performed in triplicate (*P < 0.05; **P < 0.01). Magnification, ×630 (A and B), ×400 (C).