Figure 10.

LM22A-4 ameliorates degeneration of striatal DARPP-32 neurites in BACHD mice. A, DARPP-32 immunostaining in striatum of WT (left) and BACHD mice (right) treated with vehicle (Veh; top) or LM22A-4 (LM-4; bottom) is shown. Scale bar, 10 μm. The neurites of DARPP-32-stained neurons (A, arrows) of BACHD mice tend to have a smaller surface area (B) and shorter length (C) than those of WT mice (n = 8–9 mice/group; ANOVA p = 0.04, F = 3.24; p = 0.07, Student's t test WT Veh vs BACHD Veh). Treating BACHD mice with LM-4 significantly increased the surface area and length of the neurites containing DARPP-32 (⁺⁺p = 0.005 and ⁺⁺⁺p = 0.001 vs BACHD veh, two-tailed Student's t tests).