Figure 8.

The burst–1AP pattern of firing in thalamic neurons at different time intervals between pairs of stimulation. a, Top, A train of pairs of depolarizing pulses with different ISIs was delivered at different interpair intervals (Δt) to the soma of thalamic neurons. Bottom, Examples of sequences of 10 whole-cell recordings with ISI = 100 ms delivered at Δt = 500 (from three different neurons) and 3000 ms. b, Mean PPR of APs recorded in thalamic neurons at five different Δt. PPR of APs from each cell was calculated as an average of 10 PPRs of APs in each sequence. c, Mean PPR of APs as a function of Δt from the data shown in b. Inset, Examples of sequences consisting of three whole-cell recordings with ISI = 1 s at different Δt. Note the occurrence of bursts during the second pulse at shorter but not longer Δt. d, Image of a thalamic neuron with recording pipettes at whole-cell and cell-attached configurations. Dotted lines outline the tip of a cell-attached pipette. e, Representative whole-cell and cell-attached traces at two different ISIs. Dotted line represents an arbitrary threshold for cell-attached recordings. Red dots show the number of detected APs using this threshold. Small dots show the delivery of depolarizing pulses.