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. Author manuscript; available in PMC: 2013 Nov 27.
Published in final edited form as: Mol Microbiol. 2012 Sep 11;86(3):10.1111/mmi.12004. doi: 10.1111/mmi.12004

Fig. 5.

Fig. 5

NaxD is required for the galactosamine modification of F. novicida free lipid A.

A–D. Total lipids were extracted from (A, C) wild-type F. novicida (WT) and (B, D) naxD mutant (ΔnaxD) strains in mid-log phase and (A, B) free lipid A, (C, D) undecaprenyl phosphate-N-acetylgalactosamine (GalNAc), and undecaprenyl phosphate-galactosamine (GalN) were analysed by ESI/MS in negative ion mode via direct infusion.

E. A schematic for the deacetylation of undecaprenyl phosphate-GalNAc (expected m/z = 1048.74) to undecaprenyl phosphate-GalN (expected m/z = 1006.73) is shown.

(A, E) Galactosamine is highlighted in green and (E) the acetyl group is highlighted in red.