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NaxD is necessary for deacetylation of undecaprenyl phosphate-N-acetylgalactosamine. Deacetylase activity assays using synthesized undecaprenyl phosphate-N-acetylgalactosamine (GalNAc) and (A) 0.5 mg ml−1 F. novicida wild-type (WT) membrane fraction or (B) F. novicida naxD mutant (ΔnaxD) membrane fraction were incubated at 30°C for the indicated times and then analysed using LC-ESI/MS in negative ion mode.
