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. 2013 Nov 15;27(22):2445–2458. doi: 10.1101/gad.229880.113

Figure 4.

Figure 4.

Suppression of Rad52 foci and hyperrecombination by overexpression of RNH1. (A) Rad52-YFP foci formation in W303-1A (WT), WNPL3-1D (npl3Δ), and U678-1C (hpr1Δ) strains carrying pCM189 (−RNH1) or pCM189RNH1 (+RNH1) in the absence of doxycycline. Representative microscope images are shown. Average and standard deviation of six independent experiments are shown normalized to wild-type levels. (B) Recombination analysis in the TL-lacZ system in W303-1A (WT), WNPL3-1D (npl3Δ), and U678-1C (hpr1Δ) strains under the same conditions as in A. Average and standard deviation of three to four fluctuation tests from six independent colonies for each one are shown normalized to wild-type levels. (*) P < 0.05; (**) P < 0.01 (Student's t-test).