Fig. 2.

Lipid raft disruption impairs TRPC channel function. A:Whole-cell patch clamp recordings were used to isolate currents sensitive to 25 μM SKF96365, a TRPC inhibitor. The drug sensitive current was plotted as a function of applied voltage. In normal serum conditions, D54MG cells displayed small, linear SKF-sensitive currents (n=17). When lipid rafts were disrupted with either chronic serum depletion (n=16) or MβCD (n=24), D54MG cells did not display SKF-sensitive currents. Acute serum conditions (n=25) were used for MβCD treatment, but did not themselves affect SKF-sensitive currents. B: Plots of Fura-2 imaging using the SOCE protocol (see Materials and Methods Section). SOCE was significantly decreased (P<0.05) with chronic serum depletion disruption of lipid rafts (n=335) as compared to cell grown in normal serum conditions (n=341). C: Quantification of SOCE peak revealed that chronic serum depletion to disrupt lipid rafts decreased SOCE by 41±1% (P < 0.05).