Fig. 4.
Quantitative (q)PCR confirmation and analyses of selected genes. qPCR confirmed the microarray increased expression of IL-1β, Tnn, Mmp3, and Ccl7 in DA compared with congenics. We used qPCR to analyze IL-17A and IL-6 since the first was not represented in the microarray and the second had variable detection. Both IL-17A and IL-6 were had significantly increased expression in DA and reciprocally reduced in congenics. Additionally, we use qPCR to confirm the increased expression of Scd1 and Pparg in congenics compared with DA. All P values ≤ 4 × 10−8, t-test.