Protein abundance of the thiazide-sensitive cotransporter [sodium-chloride cotransporter (NCC)] and the three subunits of ENaC (α, β, and γ) in CTX (A), OM (B), and IM (C) in CON, NIF-, and NIF + SPR-treated virgin female rats. Band densities were normalized to virgin controls with virgins set at 100% and summarized as bar graphs. A one-way ANOVA with Tukey's post hoc was performed. Data are presented as means ± SE. P < 0.05 vs. CON (*), and NIF + SPR (†).