Table 1.
Mouse Type | Cell Type | Input Resistance, MΩ | Membrane Time Constant, ms | AP Base Duration, ms | AP Amplitude, mV | AP Threshold, mV | fAHP Amplitude, mV | Ih Sag, mV (trough potential, mV) |
---|---|---|---|---|---|---|---|---|
Pitx3WT | Type A | 373.8 ± 28.7 (166.3–705.8) n = 37 | 37.4 ± 2.3 (14.2–66.9) n = 37 | 1.34 ± 0.06 (0.92–1.86) n = 37 | 70.5 ± 1.8 (54.0–91.0) n = 37 | −41.1 ± 0.7 (−34.2 to −50.0) n = 37 | −17.4 ± 0.7 (−11.0 to −24.6) n = 37 | 8.9 ± 0.7 (n = 25) (at −100.1 + 0.9) (6.1–14.0) |
Pitx3WT | Type B | 167.2 ± 10.5 (81.9–253.6) n = 26 | 20.2 ± 1.8 (14.6–33.8) n = 26 | 0.73 ± 0.01 (0.61–0.89) n = 26 | 71.4 ± 1.8 (53.3–89.5) n = 26 | −43.3 ± 1.1 (−31.6 to −58.4) n = 26 | −21.5 ± 0.8 (−16.4 to −30.5) n = 26 | 4.8 ± 0.6 (n = 11) (at −97.7 + 0.9) (2.7–7.7) |
Pitx3Null | Type A | 377.6 ± 24.7 (170.4–720.9) n = 35 | 37.2 ± 2.5 (15.2–73.8) n = 35 | 1.38 ± 0.04 (0.95–1.83) n = 35 | 70.8 ± 1.4 (58.6–90.9) n = 35 | −40.2 ± 0.7 (−35.2 to −51.4) n = 35 | −17.6 ± 0.6 (−10.8 to −24.5) n = 35 | 8.8 ± 0.6 (n = 31) (at −100.1 + 0.9) (6.4–12.5) |
Pitx3Null | Type B | 171.7 ± 10.4 (96.8–286.5) n = 30 | 21.1 ± 1.3 (12.0–33.3) n = 30 | 0.72 ± 0.02 (0.51–0.88) n = 30 | 71.2 ± 1.2 (53.3–90.7) n = 30 | −43.4 ± 0.8 (−38.1 to −56.1) n = 30 | −21.1 ± 0.7 (−16.5 to −34.8) n = 30 | 4.9 ± 0.5 (n = 14) (at −98.8 + 1.2) (3.0–6.9) |
Pitx3WT vs. Pitx3Null | Type A | P > 0.05 | P > 0.05 | P > 0.05 | P > 0.05 | P > 0.05 | P > 0.05 | P > 0.05 |
Type B | P > 0.05 | P > 0.05 | P > 0.05 | P > 0.05 | P > 0.05 | P > 0.05 | P > 0.05 | |
Type A vs. type B | WT | P < 0.001 | P < 0.05 | P < 0.001 | P > 0.05 | P > 0.05 | P < 0.05 | P < 0.01 |
Homo | P < 0.001 | P < 0.05 | P < 0.001 | P > 0.05 | P > 0.05 | P < 0.05 | P < 0.01 |
Values are means ± SE for n cells. Values in parentheses indicate ranges of individual values. GP, globus pallidus; AP, action potential; fAHP, fast afterhyperpolarization; WT, wild type; Homo, Pitx3Null. P values are from unpaired t-tests.