FIGURE 2.

Time dependency and dose dependency of CORT effects on the thorn density of CA3 neurons. Thorns were analyzed along the primary and secondary dendrites of pyramidal neurons in the stratum lucidum of CA3 neurons. (A) The time dependency of CORT effects on the thorn density in CA3 neurons, after 0.5 h treatment (0.5 h), 1 h treatment (1 h), and 2 h treatment (2 h) in ACSF with 1 μM CORT. As a control, no treatment with CORT (0 h) is shown. (B) Dose dependency of CORT treatments on the thorn density. A 1 h treatment in ACSF without CORT (0 nM), with 10 nM CORT (10 nM), with 30 nM CORT (30 nM), with 100 nM CORT (100 nM), with 500 nM CORT (500 nM), and with 1 μM CORT (1 μM). Vertical axis is the average number of thorns per 1 μm of dendrite. Results are reported as mean ± SEM. The significance of CORT or drug effect was examined using the Tukey–Kramer post hoc multiple comparisons test when one way ANOVA tests yielded P < 0.05. The significance yielded **P < 0.01 *P < 0.05, **P < 0.01 to 0 h and 0 nM. For each drug treatment, we investigated 3 rats, 6 slices, 12 neurons, 12 dendrites, and 1400–1800 thorns.