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. 2013 Nov 27;7:191. doi: 10.3389/fncir.2013.00191

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Copyright © 2013 Yoshiya, Komatsuzaki, Hojo, Ikeda, Mukai, Hatanaka, Murakami, Kawata, Kimoto and Kawato.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunoelectron microscopic analysis of the distribution of GR within the mossy fiber synapses, dendrites in stratum lucidum and nuclei of pyramidal cells in CA3 region. Gold particles (arrowheads), specifically indicating the presence of GR, were localized in the pre- and postsynaptic regions (A). In dendrites, gold particles were often found in the cytoplasmic space (B). Gold particles were also localized in the nuclei (C). A search for immuno-gold labeled GR proteins was performed at least 30 synapses at CA3 region from more than 100 independent images. A 1:3000 dilution of IgG was used to prevent non-specific labeling. Pre, presynaptic region; Post, post synaptic region; Den, dendrite; Nuc, nucleus. Scale bar, 500 nm.