Table 1.

Agent variables in the IVSABM. A summary of the variables encoded in each IEC in the IVSABM. This includes membrane bound receptors which can interact with diffusible variables in the environment, as well as intracellular molecules which act as signaling intermediates and effectors.

IEC Agent Variable	Description	Effect in IVSABM
EGFR	Membrane-bound receptor	Binds EGF, leads to effectors of motility, cell spreading
TLR-4	Membrane-bound receptor	Binds DAMPs/ROS, activates MEK/ERK pathway
P2Y	Membrane-bound receptor	Binds ATP, activates PLC/PKC pathway
A2B	Membrane-bound receptor	Binds adenosine, activates PI3K pathway
TGF-β-R	Membrane-bound receptor; available only in absence of tight junctions	Binds TGF-β, leads to LM-332 deposition in SECM
Integrin-α3β1	Membrane-bound receptor; induced by wounding	Binds LM-332, activates Rac1
Integrin-α6β4	Membrane-bound receptor	Binds LM−511, activates RhoA
CD73	Membrane-bound receptor; upregulated 10-fold by HIF-1α	Converts ATP to adenosine
Intracellular kinases (MEK, ERK, PLC, PKC, PI3K, Src, Akt, SMAD)	Signaling intermediates	Lead to various pathways and effector molecules
HIF−1α	Transcription factor; induced by hypoxia	Upregulation of CD73
NFκB	Transcription factor	Leads to EGFR expression
ADAM-17	Metalloprotease	Releases membrane-bound EGF into media
FAK/Paxillin	Effectors	Form a complex to induce simulated lamellopodial protrusion
mTOR	Effector	Cell spreading
Rac1	Effector; induced by integrin- α3β1	Pro-motility
RhoA	Effector; induced by integrin- α6β4	Pro-stability