Table 1.
Comparison of Reactions of AmpDh2 and AmpDh3 with sacculus.a
| Solubleb | Insolubleb | ||
|---|---|---|---|
| AmpDh2 | Products | 3,900,000 (53%) | 310,000 (86%) |
| Partial-reaction | 3,300,000 (45%) | 50,000 (14%) | |
| Substrates | 150,000 (2%) | Not detected | |
|
| |||
| Total | 7,400,000 (100%) | 360,000 (100%) | |
|
| |||
| AmpDh3 | Products | 300,000 (100%) | 19,000,000 (93%) |
| Partial-reaction | Not detected | 1,100,000 (5%) | |
| Substrates | Not detected | 390,000 (2%) | |
|
| |||
| Total | 300,000 (100%) | 20,000,000 (100%) | |
a
Amounts are expressed as the total EIC peak areas.
b
Souble and insoluble portions were separated by centrifugation of reaction mixtures with sacculus, before digestion by mutanolysin.