TABLE 1. Probes in the Ral Signature.

Probe*	Fold Change^	Gene Symbol	Bladder Ca#	Squamous Ca#	CaP#
222043_at	4.88	CLU	(−)	(−)	(−)
203325_s_at	3.90	COL5A1	(−)	(+)
204396_s_at	3.73	GRK5
204584_at	3.73	L1CAM
212488_at	3.56	COL5A1	(−)	(+)
212489_at	3.41	COL5A1	(−)	(+)
213397_x_at	3.23	RNASE4	(−)	(−)
203845_at	3.13	KAT2B		(−)
202196_s_at	3.12	DKK3		(−)	(−)
211071_s_at	3.10	MLLT11
206924_at	3.09	IL11
205158_at	2.80	RNASE4	(−)	(−)
218625_at	2.76	NRN1
214247_s_at	2.68	DKK3		(−)	(−)
206117_at	2.65	TPM1	(−)	(+),(−)	(−)
212888_at	2.51	DICER1
202952_s_at	2.42	ADAM12		(+)
202733_at	2.40	P4HA2
213790_at	2.38	ADAM12		(+)
202743_at	2.35	PIK3R3
213005_s_at	2.33	KANK1		(−)	(−)
201506_at	2.33	TGFBI	(−)	(+)
221541_at	2.30	CRISPLD2	(−)		(−)
208792_s_at	2.30	CLU	(−)	(−)	(−)
208791_at	2.28	CLU	(−)	(−)	(−)
206116_s_at	2.27	TPM1	(−)	(+),(−)	(−)
212099_at	2.23	RHOB
222062_at	2.21	IL27RA		(+)
209822_s_at	2.19	VLDLR
210986_s_at	2.17	TPM1	(−)	(+),(−)	(−)
201505_at	2.17	LAMB1		(+)	(−)
203871_at	−2.12	SENP3
218190_s_at	−2.27	UQCR10
208756_at	−2.31	EIF3I		(+)
215113_s_at	−2.74	SENP3
221263_s_at	−2.84	SF3B5
215171_s_at	−3.04	TIMM17A		(+)	(+)
201528_at	−3.48	RPA1
204475_at	−5.05	MMP1	(+)	(+)

^*Indicated probe from the Affymetrix HG-U133A oligonucleotide microarray platform.

^{^}Average fold change comparing control siRNA treated UM-UC-3 cells to cells depleted of RalA and RalB.

^#Direction of gene expression change (+ or −) in bladder carcinoma, squamous carcinoma (of esophagus, cervix, or upper aerodigestive tract), or prostatic adenocarcinoma, compared to normal respective tissue. Direction indicated if the gene were in the top 1% of genes in ≥ 1 study of the relevant tumor type in the Oncomine database (53).