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. Author manuscript; available in PMC: 2014 Oct 24.
Published in final edited form as: Cell. 2013 Oct 10;155(3):10.1016/j.cell.2013.09.046. doi: 10.1016/j.cell.2013.09.046

Figure 2. Cxcr7 is required for Sdf1a-GFP sequestration by the primordium.

Figure 2

(A, B) Average fluorescence intensity of Sdf1a-GFP protein along the stripe of chemokine producing cells underneath primordia in embryos of indicated genotypes (B, inset, heat-shocked wild-type control embryos). (C) Sum projection of the primordium from 36 hpf embryos of indicated genotypes stained for Claudin-B and GFP. Scale bar corresponds to 10 μm. Direction of migration is to the right. Arrowheads indicate Sdf1a-GFP puncta. (D) Distribution of intensities of Sdf1a-GFP puncta in primordia of indicated genotypes. (E) Distribution of intensities of Sdf1a-GFP puncta in primordia along the anterior-posterior axis. Each dot (D and E) represents an individual punctum (red: cxcr7b−/−; cxcr7a morphant primordia; black: wild type primordia). (F) Fraction of Sdf1a-GFP found inside the primordium of the total Sdf1a-GFP in the indicated genotype. Each dot represents the fraction of Sdf1a-GFP in an individual primordium. Horizontal bars represent the mean +/− SEM. (See also Figure S1)