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. Author manuscript; available in PMC: 2014 Oct 24.
Published in final edited form as: Cell. 2013 Oct 10;155(3):10.1016/j.cell.2013.09.046. doi: 10.1016/j.cell.2013.09.046

Figure 4. Cxcr7 generates the Sdf1-signaling gradient across the primordium.

Figure 4

(A) Single confocal slices through the primordium in live 36 hpf embryos of the indicated genotypes, all carrying the Sdf1-signaling sensor. The FmemRed/FmemGreen images are inverted heat maps of the ratio. (B) Mean FmemRed/FmemGreen along the anterior-posterior axis of n≥10 primordia with 0 μm representing the front of each primordium. Red circles indicate the mean FmemRed/FmemGreen in embryos of the indicated genotype; black circles, where present, indicate the mean FmemRed/FmemGreen of wild-type embryos, heat-shocked control embryos or cxcr7 deficient control embryos. Grey bars indicate SEM. Anterior is to the left and dorsal is up in A. Scale bar is 20 μm. Note that cxcr7 deficient; tg(tubb2b:cxcr7b) embryos were injected with a low dose of cxcr7a MO mix. (See also Figures S2, S4 and S6 and supplemental discussion)