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. Author manuscript; available in PMC: 2013 Nov 27.
Published in final edited form as: DNA Repair (Amst). 2008 Jul 22;7(9):10.1016/j.dnarep.2008.06.007. doi: 10.1016/j.dnarep.2008.06.007

Fig. 4.

Fig. 4

In situ hybridization with a DIG-RNA antisense probe to mNeil3 in horizontal and sagittal brain sections from P3, 1-month- and 1-year-old mice. (A) Horizontal section through hippocampus and the subventricular zone (SVZ) of a P3 mouse. Part of the section (DG, dentate gyrus, SVZ, subventricular zone) in A is enlarged in insert. (B) Sagittal section showing the rostral migratory stream (RMS) from olfactory bulb to hippocampus in a P3 mouse. (C) Horizontal section of cerebellum in P3 mouse brain. (D) Horizontal section of 1-month-old mouse brain displaying the layer V labelling in the neocortex. (E) Horizontal section through the SVZ with plexus choroideus (PC) in a 1-month-old mouse brain. (F) Horizontal section of the neocortex in a 1-year-old mouse brain. High expression of mNeil3 was observed in SVZ, RMS, the hilar cells of the hippocampal formation and in cerebellum of P3 mouse brain. Transcripts of mNeil3 in SVZ decreased with age with few labelled cells present in 1-month mice and absence of SVZ transcripts in 1-year-old mice. In 1-month- and 1-year-old brain (but not in P3 brains) mNeil3 was detected in layer V of neocortex. No labelling was detected with the sense probe (insert in A). CP, caudatoputamen; TH, thalamus; F, fornix; PC, plexus choroideus; DG, dentate gyrus; SVZ, subventricular zone; HC, hippocampus, RMS, rostral migratory stream; OB, olfactory bulb. Scale bar, 100 μm.