Table 2.
Distinct host peptides identified by mass spectrometry bound to DENV.
| Cell/tissue expression | Protein name | Experment number | Peptide identified | Score |
|---|---|---|---|---|
| C6/36 (57 kDa) | Enolase | 1 | K.EALNLIQDAIAK.A | 45.6 |
| R.GNPTVEVDLVTDLGLFR.A | 62.1 | |||
| K.VNQIGTVTESINAHLLAK.K | 76.1 | |||
| R.SGETEDTFIADLVVGLSTGQIK.T | 76.1 | |||
| C6/36 (67 kDa) | 2 | FGLDATAVGDEGGFAPNILNNKEALDLINEAISK | 60.5 | |
| DS3 | 3 | GVLKAVTQ | 20.2 | |
| DMEB (67 kDa) | 4 | R.AAVPSGASTGVHEALELR.D | 53.2 | |
| K.NLILPVPAFNVINGGSHAGNKQAMQEFMILPTGACSFTEAMK.M | 21.7 | |||
|
| ||||
| DMEB (67 kDa) | Beta-adrenergic receptor kinase | 1 | ESQELLGSMAKK | 40.1 |
| DS3 (67 kDa) | 2 | ESQELLGSMAKK | 40.1 | |
| C6/36 (67 kDa) | 3 | AKPGAEAHPPFRQHK | 26.9 | |
|
| ||||
| C6/36 (57 kDa) | Translation elongation factor EF-1 alpha/Tu | 1 | SGDAAIVNLVPSWPLCVESFQEFPPLGR | 82.9 |
| Mori (extract) | 2 | NNPPKQAA | 21.8 | |
| IBO | 3 | K.GASDFTAQVIVLNHPGQIANGYTPVLDCHTAVIACKFAEIQQK.V R.LPLQDVYK.I |
63 | |
|
| ||||
| C6/36 (80 kDa) | Cadherin | 1 | FLIDYGSGTLELRIATK | 52 |
*Proteomic analysis was performed in protein from C6/36 cells, mosquito MGS purified by affinity chromatography (extract), or in the bands of interest excised after separation by SDS-PAGE.