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. Author manuscript; available in PMC: 2013 Nov 27.
Published in final edited form as: Eur J Immunol. 2012 Nov 27;43(1):10.1002/eji.201242697. doi: 10.1002/eji.201242697

Figure 4.

Figure 4

Different functions of Klf10 in M-BMMs and GM-BMMs. (A) Different expression patterns of IL-12p40, IL-6, and IL-10 in GM-BMMs and M-BMMs. Bone marrow cells from WT or Klf10-deficient mouse were cultured with either 20 ng/mL GM-CSF or 10 ng/mL M-CSF for macrophage polarization. On day 5, cells were stimulated with 1 μg/mL LPS. Cells were harvested at the indicated time, and the relative mRNA expression levels of IL-12p40, IL-6, and IL-10 were measured by qPCR and normalized to levels in untreated cells. (B) GM-BMMs on day 5 were pretreated with 2.5 × 10−7 M dexamethasone, 2 ng/mL TGF-β, or 10 ng/mL IL-10 for 16 h for deactivation, and then stimulated with 1 μg/mL LPS. Cells were harvested at the indicated time, and the relative mRNA expression levels of IL-12p40, IL-6, and IL-10 were measured by qPCR and normalized to those in untreated cells. (C) The expression of Klf10 in GM-BMMs from WT mouse in (B) was measured by qPCR. Data are shown as mean + SD of three samples pooled from three independent experiments. *p < 0.05, **p < 0.01; determined by Student’s t-test.