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. Author manuscript; available in PMC: 2013 Nov 27.
Published in final edited form as: Eur J Immunol. 2012 Nov 27;43(1):10.1002/eji.201242697. doi: 10.1002/eji.201242697

Figure 5.

Figure 5

Similar function of Klf11 and Klf10 in the regulation of IL-12p40 expression.(A) Overexpression of Klf10 or Klf11 reduced the LPS-induced production of IL-12 p40 and IL-6 in M-BMMs from WT and Klf10-deficient mice. M-BMMs on day 5 were transfected with pcDNA3-Klf10, pcDNA3-Klf11, or empty vector (Mock), cultured for 24 h and then stimulated with 1 μg/mL LPS. Cells were harvested at the indicated times, and relative mRNA expressions of IL-12p40 and IL-6 was measured by qPCR and normalized to levels in untreated cells (left). Supernatants from the cultures were collected and the levels of IL-12p40 and IL-6 were evaluated by ELISA (right). (B) Silencing of Klf11 expression promoted the production of LPS-induced IL-12p40 in M-BMMs from WT and Klf10-deficient mice. On day 5, M-BMMs were transfected with Klf11 SiRNA or control SiRNA, cultured for 36 h, and then stimulated with 1 μg/mL LPS. Cells were harvested at the indicated times, and relative mRNA expression of IL-12p40 and IL-6 was measured by qPCR and normalized to levels in untreated cells (left). Supernatants from the cultures were collected, and the levels of IL-12p40 and IL-6 were evaluated by ELISA (right). Data are shown as mean ± SD of three samples pooled from three independent experiments. *p < 0.05, **p < 0.01, determined by Student’s t-test.