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. 2013 Aug 2;22(25):5107–5120. doi: 10.1093/hmg/ddt365

Figure 3.

Figure 3.

COL15A1 levels modify SMC phenotype. (A) Human AoSMCs (7F4356) were transfected with siCOL15A1 or siControl and the level of COL15A1 (A) gene expression normalized to Beta-2 Microglobulin (B2M) and (B) protein expression normalized to β-actin (ACTB) were measured. A representative western blot is shown for COL15A1 and loading control β-actin. (C) COL15A1 control or knockdown cells were assayed for proliferation using the MTS assay from Promega. Data were normalized to the siControl. (D) COL15A1 control and knockdown cells were assayed for their ability to migrate through a permeable membrane in response to fibronectin. †P, paired t-test (n = 3), two-tailed.