Table III.

Effects of MAP kinases’ and NF-kB inhibitors on LPS- and E.coli K-12-induced IL-6 secretion in normal rat macrophages^a

Inhibitor b	IL-6 (ng/mg cell protein)
	LPS	E. coli K-12
PD 98059 (MEK inhibitor – MAP kinase kinase)
0 µM	10.01 ± 0.54	15.9 ± 1.22
5 µM	6.26 ± 0.36 c	11.1 ± 2.47
20 µM	5.5 ± 0.037 c	10.6 ± 1.04
50 µM	5.67 ± 0.42 c	8.27 ± 0.95 c
SB 203589 (p38 MAPK Inhibitor)
0 µM	10.01 ± 0.54	15.9 ± 1.22
1 µM	8.34 ± 0.061	9.01 ± 0.1 c
5 µM	7.72 ± 1.02	7.44 ± 0.55 c
25 µM	6.12 ± 0.025 c	6.55 ± 0.06 c
SP 600125 (JNK 1/2/3 Inhibitor)
0 µM	10.01 ± 0.54	15.9 ± 1.22
1 µM	6.51 ± 0.72 c	9.53 ± 0.93 c
5 µM	4.05 ± 0.98 c	5.02 ± 0.24 c
10 µM	3.98 ± 0.92 c	3.14 ± 0.05 c
25 µM	2.71 ± 0.37 c	2.81 ± 0.07 c
Sulfasalasine
0 µM	10.01 ± 0.54	15.9 ± 1.22
0.01mM	10.26 ± 0.3	11.69 ± 0.45
0.1mM	8.92 ± 1.65	11.34 ± 0.91
1 mM	7.87 ± 0.64	9.69 ± 1.04
PDTC (pyrrolidinedithiocarbamate)
0 µM	10.01 ± 0.54	15.9 ± 1.22
1 µM	8.76 ± 0.27	11.71 ± 0.11
5 µM	8.59 ± 0.72	10.17 ± 0.44 c
10 µM	7.83 ± 0.25 c	9.21 ± 1.01 c
25 µM	5.84 ± 0.48 c	7.81 ± 0.27 c
50 µM	3.52 ± 0.34 c	4.14 ± 0.09 c

^aData are presented as means ± S.D. of one of three separate experiments that were performed in duplicates and yielded similar results. IL-6 levels are expressed in ng per mg of cell protein.

^bCells were treated with the indicated concentrations of inhibitors for 1hr prior to a 20h stimulation with LPS (100ng/ml) or E. coli K-12 (2ng/ml).

^cp < 0.05, IL-8 levels in the presence of inhibitor vs. control (in the absence of inhibitor), Student’s t test.