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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: J Immunol. 2013 Oct 18;191(11):10.4049/jimmunol.1301019. doi: 10.4049/jimmunol.1301019

Figure 8.

Figure 8

β2AR stimulation enhances CD23 and ADAM10 expression on exosomes after priming. CD40L/IL-4 primed B cells were cultured as described in Figure 1 and B cell-derived exosomes were isolated from supernatants after 2 through 5 days. Equal amounts of protein were analyzed by Western blot for CD23, ADAM10 and CD63 for each timepoint. (A) BCA analysis of isolated exosomes. Data are presented as mean amount of protein (mg/ml) +/- SEM from 2-3 independent experiments. B-D. B) One representative blot from three independent experiments is shown, with Molecular Weight in kDa, and data are presented as the mean fold difference of CD23 (C) and ADAM10 (D) expression +/- SEM from 3 independent experiments. Significance based on an unpaired t-test. **, p= .01, compared to priming alone.